Distribution of sorcin in cell cycle.

A: Interphase and mitotic 3T3-L1 fibroblasts stained for sorcin using the mouse monoclonal antibody. DNA stained using DAPI. Note the dispersion of nuclear sorcin throughout the cell after prophase, and its concentration in the cleavage furrow of cells in late telophase before its accumulation in the midbody and its reentrance in the nucleus in cells in cytokinesis. Bars: 5 µm. B: Sorcin association with tubulin-based structures. Mitotic mouse 3T3-L1 fibroblasts simultaneously stained for sorcin, tubulin and DNA in interphase (a), metaphase (b), telophase (c) and before abscission (d). Note the association of sorcin with microtubular structures during interphase, with the poles of the mitotic spindle during metaphase, with the cleavage furrow of cells in telophase, its accumulation in the midbody and reentrance in the nucleus of cells in cytokinesis, before completion of mitosis. Observe the flanking of sorcin by tubulin. Bars: 10 µm.