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Directed differentiation of transgene-free iPSCs into hepatic progenitors is not affected by the epigenetic status of the Dlk1-Dio3 gene cluster.

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posted on 2012-12-14, 01:32 authored by Cesar A. Sommer, Constantina Christodoulou, Andreia Gianotti-Sommer, Steven S. Shen, Badi Sri Sailaja, Hadas Hezroni, Avrum Spira, Eran Meshorer, Darrell N. Kotton, Gustavo Mostoslavsky

qRT-PCR analysis of the differentiating cultures at different time points demonstrates induction of the endoderm marker Sox17 followed by an increase in expression of the liver markers AFP and albumin. Remarkably, all iPSC clones displayed an in vitro differentiation capacity comparable to ESCs regardless of the expression levels of Gtl2 and Dlk1.