Direct quantification of VACV-specific CD8+ T cells.
Splenocytes from mice infected with VACV-WR were serially diluted in splenocytes from mice infected with VACV-ΔB8R-WR and CD8+ T cell responses to the peptides B820 and A3270 were measured using three methods. (A) Data from DimerX, IFNγ-ICS and IFNγ-ICS/CD107 methods as indicated on each graph. Backgrounds determined using irrelevant peptides were subtracted from the values presented. (B) Linear regression analysis and r2 statistics for quantification of B820–specific CD8+ T cells by each method. Data are representative of two independent experiments.