Differentiation potential of double and triple KO myoblasts.

(A) Immunostaining for MHC (green) in Ad.EV and Ad.cre transduced Rb^f/f, Rb^f/f:p107^−/−, Rb^f/f:p130^−/− and Rb^f/f:p107^−/−:p130^−/− myoblast cultures at DM-2. Nuclei were counterstained with DAPI. (B) Quantification of percent multinucleated myotubes relative to total number of MHC-positive cells (myocytes plus myotubes) in Ad.EV and Ad.cre transduced Rb^f/f, Rb^f/f:p107^−/−, Rb^f/f:p130^−/− and Rb^f/f:p107^−/−:p130^−/− myoblasts at DM-2 under normoxia. Numbers within bars indicate % for the respective samples.