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Differentiation of uncommitted mesenchymal stem cells results in elevated expression of spliced up to histone transcripts.

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posted on 22.05.2013, 01:42 by Vijayalakshmi Kari, Oleksandra Karpiuk, Bettina Tieg, Malte Kriegs, Ekkehard Dikomey, Heike Krebber, Yvonne Begus-Nahrmann, Steven A. Johnsen

(A, B) hMSCs were differentiated into (A) adipocytes or (B) osteoblasts for 15 days. Expression of marker genes PPARG for adipocytes and BGLAP for osteoblasts was analyzed by qRT-PCR. Values were normalized to HNRNPK expression. Mean±SD, n = 3. (C, D) The expression of (C) spliced HIST1H2BD or (D) spliced HIST1H2AC was analyzed by qRT-PCR using same samples as in (A) and (B). To obtain relative amounts of spliced transcript its expression was normalized to (C) total HIST1H2BD or (D) HIST1H2AC expression. Mean±SD, n = 3. P-values were calculated and statistical significance is represented as follows (ns P>0.05; *P≤0.05; **P≤0.01; ***P≤0.001).