Differential steady state levels of Sox6 in WT and Cdk5^−/− mice.

The mRNA expression of Sox6 in Cdk5 WT and Cdk5^−/− mouse brain. Total RNA was extracted from WT and Cdk5^−/− mice brain on embryonic day 17 (E17) and cDNA was prepared using reverse transcription. A) Quantitative PCR of Cdk5 expression in WT and Cdk5^−/− mice brain. The Cdk5^−/− brain shows complete abrogation of Cdk5 message. B, Sox6 expression in WT and Cdk5^−/− mice brain. There is no change in the Sox6 expression in WT and Cdk5^−/− mice n = 3 and p<0.001 (C) Lysates from embryonic day 17 (E17) age-matched wild-type (WT) and Cdk5^−/− mouse (KO) brain were separated by SDS-PAGE and transferred onto nitrocellulose. Cdk5 was detected with polyclonal C-8 antibody. Sox6 was immunodetected using Sox6 specific antibody. Equal loading was confirmed by immunodetection of β-actin. Cdk5^−/− mouse brain lysates showed elevated steady state levels of Sox6 protein. D) Densitometry analysis of Sox6 expression obtained from Figure 5C. n = 3 and *p<0.001 of Sox6 expression in Cdk5^−/− brain compared to WT.