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Differential expression of miR-17, miR-20a, and miR-106b in USSC differentiating in neuronal and osteogenic lineages.

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posted on 20.01.2011, 01:31 by Hans-Ingo Trompeter, Hassane Abbad, Katharina M. Iwaniuk, Markus Hafner, Neil Renwick, Thomas Tuschl, Jessica Schira, Hans Werner Müller, Peter Wernet

USSC lines SA5/03, SA5/73, and SA8/25 were differentiated into neuronal lineage for 14 days (SA8/25: 28 days) and USSC lines SA5/73 and SA8/25 were differentiated into osteogenic lineage for 7 days. MicroRNA expression profiles were analyzed from native USSC lines as well as from USSC differentiated into both lineages using the TaqMan microRNA Megaplex array. Fold expression changes (2−ddCt-values) are given for microRNAs miR-17, miR-20a, and miR-106b. (A) Downregulation of miR-17, miR-20a, and miR-106b during neuronal lineage differentiation of USSC. Individual results from overall 5 independent neuronal lineage differentiation approaches of USSC lines SA5/03, SA5/73 and SA8/25 are given. MicroRNAs miR-17, miR-20a, and miR-106b were found consistently downregulated in all USSC lines differentiated into cells of neuronal lineage. (B) Expression of miR-17, miR-20a, and miR-106b during independent osteogenic differentiations of USSC lines SA5/73 and SA8/25. In contrast to the neuronal lineage differentiations, expression of miR-17, miR-20a, and miR-106b remained unchanged or was slightly upregulated after 7 days of osteogenic differentiation.

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