Differential Contribution of TRPA1, TRPV4 and TRPM8 to Colonic Nociception in Mice - Fig 6
(A) Visceromotor responses to colorectal distension in WT compared to TRPM8, TRPA1 and TRPV4 knockout mice. Visceromotor responses (VMR) are presented as integrated EMG (iEMG in μVxs). TRPA1 and TRPV4 but not TRPM8 knockouts demonstrate reduced pain behavior at all tested pressure levels. Baseline EMG activity was about equal in all mouse strains. (B) Accordingly, the respective pharmacological blockers for TRPA1 and TRPV4 but not TRPM8 reduced VMRs at all pressure levels at the given dosage (HC 10 mg/kg, RN 1 mg/kg, AMTB 10 mg/kg). “+”indicates significance for TRPA1-/- or HC vs. WT, “#” for TRPV4-/- or RN vs. WT (p < 0.05; 2-way ANOVA followed by post hoc LSD test). Each data point is representative for 6–10 experiments.