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Dicer-dependent processing of 7SL RNA.

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posted on 12.07.2012, 00:19 by Yong-Feng Ren, Guiling Li, Jianmin Wu, Yong-Feng Xue, Yi-Jiang Song, Lu Lv, Xue-Jiao Zhang, Kai-Fu Tang

HepG2.2.15 cells were transfected twice with siDCR or siCon. (A&B) Representative northern blot of 7SL sRNA5cd (A) and the full-length 7SL RNA (B), U6 was used as loading control. (C) The relative level of 7SL RNA determined by real-time RT-PCR, data are shown as mean ± SD from three independent experiments. (D) 12% polyacrylamide gel electrophoresis of 7SL RNA digested by the recombinant human Dicer protein.

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