Dicer-dependent processing of 7SL RNA.
HepG2.2.15 cells were transfected twice with siDCR or siCon. (A&B) Representative northern blot of 7SL sRNA5cd (A) and the full-length 7SL RNA (B), U6 was used as loading control. (C) The relative level of 7SL RNA determined by real-time RT-PCR, data are shown as mean ± SD from three independent experiments. (D) 12% polyacrylamide gel electrophoresis of 7SL RNA digested by the recombinant human Dicer protein.