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Devices for BART assays and different formats of BART data output.

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posted on 30.11.2010 by Olga A. Gandelman, Vicki L. Church, Cathy A. Moore, Guy Kiddle, Christopher A. Carne, Surendra Parmar, Hamid Jalal, Laurence C. Tisi, James A. H. Murray

(A) Original laboratory set-up for BART used in the research presented in this paper. (B–D) Later designs of custom equipment for BART assays. High-throughput CCD-camera based system for laboratory use available in 96/384-well format. (B) Exploded diagram and picture (C) of the CCD-camera-based device: 1 – light box, 2 – CCD-camera, 3- samples in standard 0.2 ml PCR tubes or 8-well strips or 96-well plate, 4 – heating block. (D) Portable diode-based device for one or two 8-well strips. (E) Graphical representation of the data for a dilution series: red – 1 ng, orange – 100 pg, green – 10 pg, blue – 1 pg, black - NTC. (F) Corresponding images of LAMP-BART reactions taken at 10 (a), 21 (b), 24 (c), 25 (d), 26 (e) and 30 min (f): top row – ChAT DNA dilution series with the decreasing amount of template 1 ng, 100 pg, 10 pg, 1 pg (left to right); bottom row – no-template control.

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