Developmental expression pattern of zebrafish wdr43 mRNA.
(A–L) Whole mount ISH images of sense and anti-sense wdr43 probes in developmentally staged zebrafish embryos. (M) RT-PCR results of wdr43 gene expression in wild type and fan mutant embryos at indicated times (hpf). fan mutant mRNA was detected at 48 and 72 hpf, as indicated by digestion with DdeI, a unique restriction site generated by the fan point mutation. (N–Q) Sectioned ISH revealed discrete wdr43 mRNA expression in neurepithelium (ne), pharyngeal arch tissues (pa), and gut (g) (arrows). (N′, P′) Higher magnification images of boxed regions in N, P, respectively. Sense controls did not exhibit staining (O, Q).