Development of seven new molecular markers in Glu-D1 region.
(A) Organization of homologous Glu-D1 regions in the common wheat variety Renan and the Ae. tauschii accession AUS18913. The diagrams illustrating the organization of the various transposon elements and genes in the two Glu-D1 regions were adapted from previously published works , . Only the structures relevant to this work are shown. The letters “a” and “b” denote distinct copies of the duplicated genes. Positions of the seven newly developed DNA markers (Xms1, Xid1, Xrj1, Xrj2, Xms2, Xrj3, and Xrj4) are indicated by filled arrowheads. The “*” and “#” symbols indicate the markers based on microsatellite or indel. The HMW-GS genes Glu-D1d-1 (1Dx5), Glu-D1d-2 (1Dy10), Glu-D1a-1 (1Dx2) and Glu-D1a-2 (1Dy12) are marked by empty arrowheads. The Wis-1 and Wis-2p insertions were unique to Renan Glu-D1. The Angela-4 element, although intact in AUS18913 Glu-D1, had its internal region deleted in Renan Glu-D1 (and thus named as Angela-4p). Compared to the solo-LTR Sabrina-3s in Renan Glu-D1, its counterpart in AUS18913 Glu-D1 underwent further sequence deletion (and thus designated as Sabrina-3sd). (B) SDS-PAGE analysis of HMW-GS subunits from 14 common varieties containing Glu-D1a, b, c, d, e or f alleles. The 1Dx and 1Dy subunit pairs (1Dx5+1Dy10, 1Dx2+1Dy12, 1Dx3+1Dy12, 1Dx4+1Dy12, 1Dx2+1Dy10, 1Dx2.2+1Dy12) encoded by different Glu-D1 alleles are indicated by Arabic numbers. (C) Amplification patterns of seven Glu-D1 markers in the 14 common wheat lines with Glu-D1a, b, c, d, e or f alleles. The size (bp) of the amplicons and the names of the markers are provided on the left and right sides of the graph, respectively.