Determination of viral loads in HIV-infected subjects (A) and HIV-infected macrophages (B).
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The viral loads in the plasma of human subjects and macrophages were determined by analyzing HIV RNA using q-RTPCR and p24 using ELISA, respectively. The data for in vitro assay (B) represents mean of multiple analysis of p24 titer obtained from HIV-infected macrophages from three different donors. The p values (# and ** represent p≤0.1 and p≤0.01, respectively) are calculated using one way-ANOVA and presented in the graph.