Determination of the subcellular localization patterns of Lvcaspase2 (A), Lvcaspase3 (B), Lvcaspase4 (C) and Lvcaspase5 (D) using confocal microscopy.

Drosophila S2 cells were transfected with pAC5.1-Lvcaspase2-5-GFP. At 36 hours post-transfection, cells on the cover slips were washed twice with PBS, fixed using Immunol Staining Fix Solution (Beyotime, China) and stained with Hoechst 33258 Solution (Beyotime, China). The cells were observed using a Leica laser scanning confocal microscope as previously described [30,31,36]. .