Determination of anti-proliferation IC50 value of honokiol. hRMVECs were treated with different concentrations of honokiol (0.4–180 µM of honokiol) for 24 h.
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The number of viable cells was calorimetrically calculated using the Premixed WST-1 Cell Proliferation Reagent by detecting the amount of formazan formed at 450 nm. Plotting the absorbance at 450 nm vs. log10 value of honokiol concentrations yielded the anti-proliferation IC50 value of 57 µM for honokiol.