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Determination of anti-proliferation IC<sub>50</sub> value of honokiol. hRMVECs were treated with different concentrations of honokiol (0.4–180 µM of honokiol) for 24 h.

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posted on 2014-11-25, 03:01 authored by Divya Teja Vavilala, V. K. Chaithanya Ponnaluri, Debolina Kanjilal, Mridul Mukherji
<p>The number of viable cells was calorimetrically calculated using the Premixed WST-1 Cell Proliferation Reagent by detecting the amount of formazan formed at 450 nm. Plotting the absorbance at 450 nm vs. log<sub>10</sub> value of honokiol concentrations yielded the anti-proliferation IC<sub>50</sub> value of 57 µM for honokiol.</p>

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    Magnolia plantspigment epithelialneovascular diseasespostnatal dayHIF pathwayintraperitoneal injectionchromatin immunoprecipitation experimentsCell linesVEGF promoterP 17.RPE cellshonokiol manifestsHIF inhibitionhealth benefitsretinopathy mouse modelgrowth factorangiogenesis assaysOcular Neovascular Diseases Pathological activationischemic diseasescrude extracts

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