Detection of p23 and CP of HCRSV in newly emerged wild type (wt), (H to A) and (K, R to A, A) inoculated kenaf plants at 19 days post inoculation (dpi), respectively.
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(A) Both p23 and CP genes were detected in the newly emerged leaves of plants infected with two mutants (H A) and (K, R A, A) at 19 dpi using RT-PCR. Actin gene was used as a loading control. The band intensity of each gene transcript from RT-PCR results was quantified using ImageJ. (B) Detection of HCRSV-CP protein in the wt, mutants H to A and K, R to A, A in the newly emerged kenaf leaves at 19 dpi. The CP expression level was below detection level for the two mutants.