Detection of p23 and CP of HCRSV in newly emerged wild type (wt), (H to A) and (K, R to A, A) inoculated kenaf plants at 19 days post inoculation (dpi), respectively.
(A) Both p23 and CP genes were detected in the newly emerged leaves of plants infected with two mutants (H A) and (K, R A, A) at 19 dpi using RT-PCR. Actin gene was used as a loading control. The band intensity of each gene transcript from RT-PCR results was quantified using ImageJ. (B) Detection of HCRSV-CP protein in the wt, mutants H to A and K, R to A, A in the newly emerged kenaf leaves at 19 dpi. The CP expression level was below detection level for the two mutants.