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Detection of intratumoral CTLs.

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posted on 20.02.2013, 20:48 by Jialin Sun, Lina Zhao, Lijie Teng, Feng Lin, Hongna Zhang, Zhengnan Li, Qiang Gao

Small brown spots around S180 tumor cells indicated antibody-reactive T cells. (A) CD4-positive cells detected by rabbit CD4-specific polyclonal antibody. (B) CD8 positive cells detected by rabbit CD8-specific polyclonal antibody. (C) SEA-reactive T cells. Sections were incubated with a SEA solution, followed by mouse polyclonal anti-SEA IgG fraction and anti-mouse secondary antibody. Microscope magnification: ×400 in A to C.

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