Detection of interaction between hGBP1-K51A and NS1.
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H1299 cells were transfected with plasmid Myc-hGBP1-wt or Myc-hGBP1-K51A in the presence of plasmid Flag-NS1-wt. Transfectants were collected after 36 h and immunoprecipitated with anti-Myc and anti-Flag antibodies. IP, immunoprecipitation. IB, western blot.