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Detection of M. catarrhalis and NTHI in biofilms recovered from the chinchilla middle ear.

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posted on 20.02.2013, 03:17 by M. Elizabeth Brockson, Laura A. Novotny, Joseph A. Jurcisek, Glen McGillivary, Martha R. Bowers, Lauren O. Bakaletz

Immunofluorescence image of a biofilm incubated with rabbit-anti NTHI outer membrane protein P5 (green), mouse anti-M. catarrhalis PilA (red), and stained with DAPI to label DNA (blue). A) Merged image, B) M. catarrhalis, C) NTHI, D) serial section control demonstrating no labeling of either M. catarrhalis or NTHI. Scale bar = 5 µm.