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Depicted is a vector map of shuttle vector pBSU101 that was used in this study to label different gram-positive bacteria with EGFP.

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posted on 2013-02-20, 17:17 authored by Simone Aymanns, Stefanie Mauerer, Ger van Zandbergen, Christiane Wolz, Barbara Spellerberg

The multiple cloning site (MCS), the different origins of replication (for use in gram-positive hosts, oriR pAMß1, and gram-negative bacteria, oriR pUC) and the spectinomycin (spc) resistance gene are indicated. The plasmid is a derivative of pAT28. Expression of EGFP in pBSU101 is controlled via the promoter region of the CAMP-factor gene cfb of Streptococcus agalactiae. The plasmid carries the promoter region and the ATG startcodon of cfb, resulting in a translational fusion of the first 7 amino acids of CFB to the N-terminus of EGFP as depicted. Between the CFB fragment and the start of EGFP a small spacer of 8 amino acids is found that does not appear to disturb efficient EGFP expression.

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