Demonstration HmuY-ferrihaem complex formation after exposure of HRgpA-induced methaemoglobin to HmuY.
Before exposure, black line; 24 h after exposure, red line. For clarity, only the initial and final spectra are shown. HRgpA and HmuY were used at 0.4 and 16 µM, respectively. Starting concentration of haemoglobin was 16 µM (with respect to haemoglobin subunit) and comprised 77% methaemoglobin.