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Deletions suppressing cell lysis in Δura4 strains grown on YPD medium.

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posted on 04.11.2015, 06:21 authored by Kohei Nishino, Misaki Kushima, Yuzy Matsuo, Yasuhiro Matsuo, Makoto Kawamukai

(A) L972 (ura4+), UMP31 (Δura4), KT35 (ura4-D18), and indicated deletion mutants (Bioneer deletion library ver4.0) were grown for 12 h, then spotted onto YPD medium and incubated for 3 days at 30°C. For the alkaline phosphatase assay, YPD plates were overlaid with a phosphatase assay solution containing 50 mM glycine-NaOH (pH 9.8), 1% agar, and 2.5 mg/ml of BCIP for 30 min. (B) Cells were also observed by microscopy after 3 days of growth on YPD. Five examples of mutants exhibiting different levels of lysis suppression are shown. Bar: 10 μm.