Deletion of Ink4a/Arf rescues oval cell expansion defects in Bmi1−/− mice.
H&E staining (A) and A6 and CK19 immunostaining (B) on Bmi1+/+; Ink4a/Arf+/+, Bmi1−/−; Ink4a/Arf+/+ and Bmi1−/−; Ink4a/Arf−/− DDC treated mouse livers. Representative images of three independent experiments are shown. (C) Quantification of A6 and CK19 staining using ImageJ software. At least 3 animals in each group were assayed. ***P<0.001 by Student's t test.