Dehydrogenase/Oxidase ratios of kcat/Km of POx variants compared to the wild-type.
figureposted on 2014-10-08, 02:52 authored by Dagmar Brugger, Iris Krondorfer, Christopher Shelswell, Benjamin Huber-Dittes, Dietmar Haltrich, Clemens K. Peterbauer
Dehydrogenase activity was determined using DCPIP (light grey bars), 1,4-BQ (dark grey bars) and Fc+ (mid grey bars) as electron acceptors, oxidase activity was determined by following oxygen consumption. Wild-type activities were set at 100%. D-glucose was used as electron donor at 100 mM. Results are the mean of at least duplicate independent experiments and error bars correspond to standard deviations. The diagram was prepared using SigmaPlot 11.
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Regioselective OxidationFADH 2dehydrogenase activityferricenium ionelectron acceptoroxygen activationN 593CVariants T 166R Q 448H L 545C L 547Relectron acceptorsalternative electron acceptors DCPIPmicrotiter plate screening assayschelated metal ions showH 2Ooxidase activityposition C 2GMC familyoxidative damagescrystal structureH 2O productionoxygen reactivity