Deficiency ED5438 uncovers the <i>FoxP³⁹⁵⁵</i> self-learning phenotype.

<p><b>a</b>, Genomic region of <i>dFoxP</i> gene. The deficiency deletes all exons of the <i>dFoxP</i> locus up until the 5-SZ-3955 insertion, which was used to generate the deficiency, as well as 52 upstream genes. ED5438 leaves the downstream gene <i>hyperplastic disks</i> (<i>hyd</i>) intact. <b>b</b>, Operant self-learning performance indices in a two-minute test with the heat permanently switched off immediately after eight minutes of training showed a significant impairment of <i>FoxP³⁹⁵⁵</i>/ED5438 flies compared to control animals in which either the deficiency or a Canton S chromosome was crossed over the 5-SZ-3955 insertion (Kruskal Wallis ANOVA, H(2, N = 52) = 10.13; p<0.007; two-sided, Bonferroni-corrected post-hoc p-values indicated in the graph).</p>