Data from lower-titer injections. A.
SYBR-green PCR analysis of Pgc-1α and Pgc-1α target gene mRNA levels after saline treatment in mice microinjected unilaterally into the right SN with AAV2/10-FHA-Pgc-1α or AAV2/10-mCherry. mRNA levels were normalized to 18S and fold-change gene expression was calculated relative to the contralateral SN using the ΔΔCt method. Data were analyzed using a paired t-test (n = 6). B. Striatal Th immunoreactivity data for the contralateral side (Cntrl) compared to the ipsilateral side microinjected in the SN with AAV2/10-FHA-PGC-1α. Ipsilateral and contralateral striatal Th immunoreactivity within each treatment group was analyzed using a paired t-test (n = 7). C. Mean levels of striatal DA per mg of protein in the ipsilateral and contralateral (cntrl) striatum of mice microinjected unilaterally with a lower titer of AAV2/10-FHA-PGC-1α. Data were analyzed by paired t-test (n = 5). D. DA turnover in each treatment group. Data were analyzed by paired t-test for within group comparisons and Student’s t-test for between group comparisons. E. SYBR-green PCR analysis of dopaminergic markers. Fold change in gene expression relative to the contralateral side was calculated as before. Data were analyzed using a one-sample t-test to determine if fold-change gene expression differed from 1 (n = 5). F. SYBR-green PCR analysis of Pitx3 and Nurr1 expression (n = 5). Fold change was calculated and data were analyzed as in E.