D, L-Sulforaphane (SFN) causes cleavage of Notch1, Notch2, and Notch4 proteins in PC-3 and LNCaP cells.

Immunoblotting for cleaved and full-length Notch1, Notch2, and Notch4 using lysates from (A) PC-3, (B) LNCaP, and (C) LNCaP-C4-2B cells after 8-, 16-, or 24-hour treatment with DMSO or SFN (10 or 20 µM). Blots were stripped and re-probed with anti-actin antibody as a loading control. Immunoblotting for each protein was done at least twice using independently prepared lysates. Numbers above band represent changes in protein levels relative to corresponding DMSO-treated control.