Figure_5.tif (265.94 kB)

D-Glucose/D-Galactose (solid lines) and GalGlc/GalGal (dashed lines) ratios (A); GalGlc/GalLac (solid lines) and GalGal/GalLac (dashed lines) ratios (B) during lactose conversion by B. breve β-gal I (close symbol) and β-gal II (open symbol).

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posted on 04.08.2014, 03:13 by Sheryl Lozel Arreola, Montira Intanon, Jasmina Suljic, Roman Kittl, Ngoc Hung Pham, Paul Kosma, Dietmar Haltrich, Thu-Ha Nguyen

The reactions were performed at 30°C at an initial lactose concentration of 200 g L−1 in sodium phosphate buffer (pH 6.5) and 1 mM MgCl2 with 1.0 ULac mL−1 β-gal I or 2.5 ULac mL−1 β-gal II.