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DTH response (mM) to SLD as footpad swelling on days 45, 60 and 90 p.c.

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posted on 24.01.2014 by Reema Gupta, Vikash Kumar, Pramod Kumar Kushawaha, Chandradev Pati Tripathi, Sumit Joshi, Amogh Anant Sahasrabuddhe, Kalyan Mitra, Shyam Sundar, Mohammad Imran Siddiqi, Anuradha Dube

(A); NO production (µM) to LPS (B) and rLdEno/rLdAld (C) in the naïve macrophages co-incubated with supernatants of lymphocytes isolated from rLdEno/rLdAld vaccinated hamsters in comparison to the BCG vaccinated, infected controls, and uninfected normal hamsters on days 45, 60, and 90 p.c. The level of nitrite in the culture supernatants was assessed was assessed by Griess reagent. LTT response to ConA (D) and rLdEno/rLdAld (E) stimulation in vaccinated hamsters, BCG vaccinated, infected controls and uninfected normal hamsters. Results are means ± SE for 3–4 hamsters per group done in duplicate and are representative of two independent experiments with similar results. Significance values indicate the difference between the vaccinated group and infected group (*, p<0.05; **, p<0.01; and ***, p<0.001).

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