DNA methylation and histone modifications regulate WUS transcript levels.
A) Transcript levels of WUS in calli of the wild type (Ws) and the mutant met1. B) Transcript levels of WUS in calli of the wild type (Ler) and the mutant kyp-2. C) Transcript levels of WUS in calli of the wild type (Col) and the mutants, hac1-3, hac1-5, jmj14-1 and jmj14-2. Total RNAs were isolated from calli of wild type (Ws, Ler and Col) and various mutants (met1, kyp-2, jmj14-1, jmj14-2, hac1-3 and hac1-5) cultured on SIM at the indicated time points, respectively. WUS transcript levels were quantified by qRT-PCR. The results are shown as mean values of three biological replicates with standard errors. The relative expression level of WUS gene, corresponding to the expression level of TUBULIN2, was calculated using the comparative C(T) method. After incubating on CIM for 20 days (S0), some of the calli were transferred onto SIM for further induction for 4 days (S4) and 6 days (S6), other calli were still cultured on CIM as controls (C24, C26). C16, C24, C26 indicated that pistils as explants were cultured on CIM for 16 days, 24 days and 26 days, respectively.