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DNA Replication with respect to soft substrates.

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posted on 22.10.2013, 03:14 by Morgane Rabineau, Leyla Kocgozlu, Denis Dujardin, Bernard Senger, Youssef Haikel, Jean-Claude Voegel, Jean-Noel Freund, Pierre Schaaf, Philippe Lavalle, Dominique Vautier

A) BrdU visualized by indirect immunofluorescence of SW480 cells 4h post-synchronization on glass, E50 and E20; scale bar: 10 µm. B) Percentage of cells with nuclear BrdU determined on 3 pooled independent experiments. Fisher Exact Test shows that the proportion of cells with nuclear BrdU E50 (108/170) is significantly smaller than that on glass (400/400, p < 0.001) and the proportion on E20 (35/150) is significantly smaller than that on E50 (p < 0.001). The error bars represent 95% confidence intervals.

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