DNA Replication with respect to soft substrates.
A) BrdU visualized by indirect immunofluorescence of SW480 cells 4h post-synchronization on glass, E50 and E20; scale bar: 10 µm. B) Percentage of cells with nuclear BrdU determined on 3 pooled independent experiments. Fisher Exact Test shows that the proportion of cells with nuclear BrdU E50 (108/170) is significantly smaller than that on glass (400/400, p < 0.001) and the proportion on E20 (35/150) is significantly smaller than that on E50 (p < 0.001). The error bars represent 95% confidence intervals.