DHS “R” mediates retinoic acid-induced OTX2 repression.
(A) DNase sensitivity of DHS “R” in three OTX2-expressing (D283, D341, D721) and two OTX2-nonexpressing (D324, UW228) medulloblastoma cell lines. (B) Luciferase assays measuring enhancer activity of DHS “R” following treatment with 2 µM all-trans retinoic acid (ATRA) for 24 hours. (C) OTX2 mRNA level as determined by qPCR of medulloblastoma cells treated with 2 µM all-trans retinoic acid (ATRA) and/or 35 µM cycloheximide (CYHEX) for 8 hours. *p<0.05 relative to DMSO, **p<0.01 relative to DMSO, Student's t-test. Error bars indicate standard deviation.