DC stimulation depends on ICAM-1-LFA-1 signals and membrane-bound TNF-α on MCs.
(A) The expression of ICAM-1 on stimulated or non-stimulated BMDCs. (B, C) The expression levels of CD40, CD80, and CD86 on BMDCs co-cultured with BMMCs with isotype control Ab (Iso), anti-ICAM-1 Ab (IC), or anti-TNF-α Ab (TNF) (B), or co-cultured with BMMCs that were pretreated with or without wortmannin (WM) or H89 (C). (D) BMMCs expressing membrane-bound TNF-α in the presence of absence of BMDCs with or without stimulation with 100 ng/ml of LPS and 50 ng/ml of CCL21 for 1 h. All data are presented as the mean ± SD and are representative of three experiments. *, P<0.05.