DACT1 is highly expressed in human colon cancer cells.
(A) DACT1 mRNA levels were assayed using quantitative real-time RT-PCR analysis. Samples of colon adenocarcinoma (T, white bars) and normal-appearing control mucosa (N, black bars) were analyzed in six cases of colon cancer. (B) Expression of DACT1 protein in human colon adenocarcinoma (T) and normal-appearing control mucosa (N) in six cases, as determined by Western immunoblotting analysis. The expression of GAPDH was used as the loading control.