Cyr61 and β-catenin protein expression in tissue samples are detected by immunohistochemistry.
(A), A-1: Expression of Cyr61 in normal liver tissues was negative (400×); Expression of Cyr61 in well-differentiated HCC (A-2) was higher than in poorly differentiated HCC (A-4) (400×); A-3: Expression of Cyr61 was positive in HCC adjacent tissue (400×); A-5: Expression of Cyr61 in well-differentiated tumor regions (W) was higher than poorly differentiated areas (P) at the same section (100×); A-6: Overexpression of Cyr61 in hepatic cirrhosis of adjacent cancer areas (C), and loss of expression in HCC (T) (200×); W, well-differentiated tumor; P, poorly differentiated tumor; C, hepatic cirrhosis; T, tumor. (B), B-1: Expression of β-catenin in normal liver tissues was negative; B-2: Expression of β-catenin in tumor tissue; B-3: Expression of β-catenin in HCC adjacent tissue. (C), Correlation between Cyr61 and β-catenin protein expression levels. r = 0.793, P<0.01.