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Culturing CLL cells on MMP-9 modulates Bcl-2 family proteins in response to ATO, preventing downregulation of Mcl-1, Bcl-xL and Bcl-2.

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posted on 23.06.2014, 02:54 by Irene Amigo-Jiménez, Elvira Bailón, Estefanía Ugarte-Berzal, Noemí Aguilera-Montilla, José A. García-Marco, Angeles García-Pardo

(A,B) 10×106 primary CLL cells in RPMI/0.1% FBS were incubated on BSA- or 150 nM MMP-9-coated wells for 1 h prior to adding 3 µM ATO or vehicle. After 24 h, cell were lysed and the indicated proteins (A) and ratios (B) analyzed by Western blotting as explained. * or #P≤0.05; ** or ##P≤0.01; *** or ###P≤0.001. Symbols are: *, CLL cells on BSA vs CLL cells on MMP-9; #, ATO-treated cells compared to their respective untreated controls.