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CpG and mitochondrial DNA activate hepatocytes and macrophages.

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posted on 11.09.2015 by Pieter J. Bakker, Angelique M. Scantlebery, Loes M. Butter, Nike Claessen, Gwendoline J. D. Teske, Tom van der Poll, Sandrine Florquin, Jaklien C. Leemans

The hepatocyte cell line AML12 (A,B) (n = 3) and macrophage cell line RAW 264.7 (C,D) (n = 3) were stimulated with 25 uM CpG-oligonucleotides (CpG) (A,C) or 50 ng/ul mitochondrial DNA (mtDNA) (B,D) for 24 hours and subsequently KC or TNF-α was measured in the supernatants of respectively AML12 hepatocytes or RAW 264.7 macrophages. Two group comparisons were performed using unpaired students t-test where p < 0.05 = *, p < 0.01 = ** and p < 0.001 = ***.