Correlations between FSH and PDE3A.
(A) Inhibiting PDE3A blocked the effect of FSH on maturation. COCs were cultured in HX medium containing 50 IU/L FSH. Cilostamide (cilo) was added to one set of cultures 3, 4, 5, 6, 7, or 8 h after the start of culture. GVBD was assessed at 22 h (gray bars). The other set of cultures was examined at different intervals up to 8 h (black bars). Cilostamide blocked further meiotic maturation at each time point at which it was administered. GVBD percentage of the controls is indicated by the box. (B) Inhibiting PDE3A didn't affect cumulus expansion induced by FSH. Cumulus expansion was detected at the end of 22 h culture. HX, control; FSH, 50 IU/L FSH; FSH 3 h+Cilo, Cilostamide (cilo) was added to culture 3 h after the start of FSH treatment. FSH 8 h+Cilo, Cilostamide (cilo) was added to culture 8 h after the start of FSH treatment. Scale bar, approximately 50 µm. (C) After stimulation with FSH for 6 h, oocyte PDE3A activity significantly increased. Means marked with asterisks are significantly different (*P<0.05, **P<0.01).