Correlations between FSH and MAPK and PKAI in oocytes.
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(A) Activation of MAPK in the oocytes of COCs was required for FSH-induced meiotic maturation. DOs from the COCs primed with FSH for 3, 4, 5, or 6 h were transferred to fresh HX medium containing the specific MAPK inhibitor U0126 and were further cultured for a total 22 h. U0126 significantly inhibited GVBD in DOs. Means marked with asterisks are significantly different (*P<0.05, **P<0.01). (B) FSH-activated MAPK in oocytes of COCs. Oocytes of COCs were collected after treatment with FSH for 0, 2, 4, 6, or 8 h for Western blot analysis of MAPK phosphorylation. (C) PKAI activation inhibited FSH-induced MAPK phosphorylation in oocytes of COCs. Oocytes from COCs were collected 8 h after treatment for Western blot analysis of MAPK phosphorylation. DOs from 100 COCs were collected at various time points for Western blotting. The blot shown is representative of three experiments.