Figure_7.tif (435.53 kB)

Correlations between FSH and MAPK and PKAI in oocytes.

Download (0 kB)
figure
posted on 13.09.2012 by Junxia Li, Guankun Mao, Guoliang Xia

(A) Activation of MAPK in the oocytes of COCs was required for FSH-induced meiotic maturation. DOs from the COCs primed with FSH for 3, 4, 5, or 6 h were transferred to fresh HX medium containing the specific MAPK inhibitor U0126 and were further cultured for a total 22 h. U0126 significantly inhibited GVBD in DOs. Means marked with asterisks are significantly different (*P<0.05, **P<0.01). (B) FSH-activated MAPK in oocytes of COCs. Oocytes of COCs were collected after treatment with FSH for 0, 2, 4, 6, or 8 h for Western blot analysis of MAPK phosphorylation. (C) PKAI activation inhibited FSH-induced MAPK phosphorylation in oocytes of COCs. Oocytes from COCs were collected 8 h after treatment for Western blot analysis of MAPK phosphorylation. DOs from 100 COCs were collected at various time points for Western blotting. The blot shown is representative of three experiments.

History

Licence

Exports

Logo branding

Keyword(s)

Licence

Exports