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Correlations between E. coli effects on spermatozoa.

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posted on 16.12.2013, 03:10 by Arcangelo Barbonetti, Maria Rosaria Caterina Vassallo, Benedetta Cinque, Silvia Filipponi, Paola Mastromarino, Maria Grazia Cifone, Sandro Francavilla, Felice Francavilla

After 3 h exposure to E. coli (1.5×106 CFU/mL) in the Transwell system, the increase (%) in sperm mitochondrial generation of reactive oxygen species (ROS) and membrane lipid peroxidation as well as the decrease (%) in mitochondrial membrane potential (ΔΨm) and sperm motility were evaluated with respect to the untreated samples. The percentage of increment/decrement and not crude values were analyzed in order to overcome masking effects due to the inter-donors variability.