Construction of sequence libraries enriched for capped sRNAs.
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posted on 2014-07-31, 03:23 authored by Rehab F. Abdelhamid, Charles Plessy, Yoshio Yamauchi, Masato Taoka, Michiel de Hoon, Thomas R. Gingeras, Toshiaki Isobe, Piero CarninciStep-by-step graphical summary of the construction of the sRNA libraries. The bottom line indicates the library name; in the manuscript, these names are emphasized in italics. The key methods are immunoprecipitation for the IP K121 and IP control libraries, TAP (or mock) treatment for the Capped sRNA and sRNA libraries, and CAP Trapper for the sRNA CAGE libraries.
