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Consequences of Altered Matrix Production

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posted on 2013-02-21, 13:14 authored by Mark R Grant, Keith E Mostov, Thea D Tlsty, C. Anthony Hunt

Altered matrix production causes loss of growth control in simulated culture conditions. Axioms 4 and 5 were replaced with a new one (an example of which is shown in the insert) that allowed for de novo matrix production in any environment consisting of free space and cell neighbors (Alteration 3). This sequence of illustrations of typical simulation outcomes shows the consequences of Alteration 3 on cyst formation in simulated embedded culture. The behavior of a pcell, when formed, was not influenced by the altered axiom, only the behavior of cells. As in Figure 11, cell shading is different to identify that an altered axiom set was used. Spaces are shaded as defined in Figure 8. At simulation cycle 0 a cell is added to a matrix-filled grid. At the conclusion of cycle 4, a small cluster had formed containing two pcells. By cycle 8 a distinct pattern of unstable growth was evident, with matrix- and free space–containing, lumen-like spaces within clusters of cells. That trend was still evident at cycle 16; the outer edge contained a few pcells. Thereafter, there was no growth.