Confocal microscopy of PCI binding to untreated and Ct cells.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Jurkat cells (A) and U937 cells (B) were incubated with 250 nM PCI-Cy3 (red) and in some experiments also with 75 nM Annexin V-FITC (Aii, Bi, green). Nuclear staining was done with Hoechst (blue). Confocal microscopy pictures show Ct Jurkat cells (Ai) and untreated Jurkat cells (Aii) in two different magnifications (40×, n.a. 1.3 oil objective; 100×, n.a. 1.3 oil objective). Data in (B) show z-stack galleries of 1µM slice thickness representing a Ct U937 cell (Bi) and an untreated U937 cell (Bii) (63×, n.a 1.4 oil objective). The scale bars represents a length of 5µm. Picture processing was done in LSM Image browser software (Carl Zeiss).