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Confocal immunofluorescent analysis showing MICA/B staining.

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posted on 13.09.2013 by Yessica L. Allegretti, Constanza Bondar, Luciana Guzman, Eduardo Cueto Rua, Nestor Chopita, Mercedes Fuertes, Norberto W. Zwirner, Fernando G. Chirdo

A.- sample from an untreated CD pediatric patient with mild enteropathy showing the MICA/B expression (red) in enterocytes. SYTO® 13 (Green Fluorescent Nucleic Acid Stain) was used to stain nuclei. B.- MICA/B staining in an intestinal section from the same patient after two years on a gluten-free diet. C.- healthy non-celiac control patient. D. IC incubated in a section corresponding to sample A. (Magnification 63×). E.- Flow cytometric analysis for surface and intracellular expression of MICA/B in epithelial CD3 cells of a representative paediatric patient. F.- Flow cytometric analysis for surface and intracellular expression of MICA/B in epithelial CD3 cells of duodenal samples from adult CD patients.

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