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Concentration-dependent effect of ethanol on mitochondrial function, and expression of the PGC-1α in SH-SY5Y cells.

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posted on 2014-08-06, 03:19 authored by Zilong Liu, Yongping Liu, Rui Gao, Haixia Li, Tiffany Dunn, Ping Wu, Robert G. Smith, Partha S. Sarkar, Xiang Fang

The SH-SY5Y cells were treated with various concentration of ethanol for 24 hours, and MTS activity and intracellular ATP level were measured to assess mitochondrial function (Panel A & B). Total protein was isolated from the ethanol-treated and control cells and expression of PGC-1α was measured by Western blot analysis, and a representative Western blot is shown in Panel C. The protein density in each Western blot was quantitatively analyzed, and is shown in Panel D. Mean ± SE, n = 3, *P<0.05 vs. control, **P<0.01.