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Competitive growth assay of wild type and ΔphoB B.

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posted on 15.01.2013 by Shin Wakimoto, Haruyuki Nakayama-Imaohji, Minoru Ichimura, Hidetoshi Morita, Hideki Hirakawa, Tetsuya Hayashi, Koji Yasutomo, Tomomi Kuwahara

fragilis strains in vitro (A) and in vivo (B). These strains were equally mixed and inoculated into GAM broth, mice intestines or the peritoneal cavity. After periodical sampling from liquid culture, feces and abscesses, samples were spread on GAM agar plates, and the population levels of wild type and ΔphoB B. fragilis strains were evaluated by discriminating colony PCR. The data were expressed as wild type/ΔphoB mutant ratios. The ratios of wild type and phoB mutant strains in the intestine and abscess were compared by Chi-square test.

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