Comparison of the secretion competency via plasmid-encoded overexpression of FlgM.
(A) FlgM was secreted to the supernatant in the HMS174(DE3) strain. (B) In contrast, in the newly generated HMS174(DE3)ΔinsAB araBAD-flhDC no FlgM could be detected in the supernatant. (C) The ability to secrete FlgM was recovered in the HMS174(DE3)ΔinsAB araBAD-flhDC strain when plasmid-encoded flhDC was co-expressed. SDS-PAGE, M Fermentas PageRuler Prestained, -I whole cell sample without induction of recombinant protein expression, +I whole cell samples with induction of recombinant protein expression, C cytoplasmic fraction, Sn supernatant;