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Comparison of the effects of CHIR99021 and lithium chloride on trophoblast adhesion molecule expression.

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posted on 12.08.2015, 02:53 by Priyadarsini Kumar, Twanda L. Thirkill, Jennifer Ji, Louise H. Monte, Gordon C. Douglas

Trophoblasts were incubated in the presence of 3 μM CHIR99021 or 20 mM lithium chloride for 7 days. (A) Phase contrast image of cells at 7 days. Note the absence of spindle-shaped cells in the presence of CHIR99021 and their presence when trophoblasts were incubated with lithium chloride. (B) Expression of adhesion molecules was assessed by Western blot as described in Methods. The graphs below the Western blot show densitometric quantitation of protein bands (n = 2). C, CHIR99021; L, lithium chloride.

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