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Comparison of influenza virus recovery in conjunctival wash samples following ocular inoculation of ferrets.

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posted on 2013-02-20, 08:17 authored by Jessica A. Belser, Kortney M. Gustin, Taronna R. Maines, Mary J. Pantin-Jackwood, Jacqueline M. Katz, Terrence M. Tumpey

Ferrets were inoculated by the ocular route with 106 EID50/ml of each virus shown. Viral titers were measured in conjunctival washes (CW) collected on indicated days following serial titration in eggs; endpoint titers are expressed as mean log10 EID50/ml plus standard deviation (left y-axis and bars). Relative viral RNA copy number in conjunctival washes was determined by real-time PCR using a universal M1 primer and extrapolated using a standard curve based on samples of known virus (right y-axis and lines). The limit of virus detection was 101.5 EID50/ml. †, no ferrets survived until day 9 p.i. R-squared values are shown for those viruses where a statistically significant (p<0.05) correlation between viral titer and viral RNA copy number exists. NS, not significant.


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